Blood Buffer Essay

Objectives: After completing this exercise and reading the corresponding material in your text, you should be able to 1. Prepare a wet mount slide 2. Identify structures described in this lab on slides 3. Cite examples of the wide diversity of cell types 4. Relate differences in structure among cells to functional differences Introduction Structurally and functionally, all living things share one common feature: all living organisms are composed of cells. The development of this concept began with Robert Hooke’s seventeenth-century observation that slices of cork were made up of small units he called â€Å"cells.† Over the next 100 years, the cell theory emerged. It was formally (and independently) presented by Schleiden and Schwann in 1839, and clarified by Virchow a few years later. This theory has three principles: (1) All organisms are composed of one or more cells. (2) All life processes derive from the activities of cells. (3) All cells arise from preexisting cells. Living organisms are composed of one or many cells, and every activity that occurs in a living organism is ultimately related to metabolic processes in cells. Understanding the processes of life requires an understanding of the structure and function of the cell. Although cells vary in organization, size, and function, all share certain structural features. All are enclosed within a plasma membrane, defining the boundary of the living material. All contain a region of DNA (deoxyribonucleic acid), which forms the genetic code. Inside of the plasma membrane is watery cytoplasm, and the chemical processes inside the cell occur within this watery medium. Upon microscopic examination, we see there are two basic types of cells, prokaryotic and eukaryotic, see the table below to see a comparison of the two cell types. For these following exercise you should use the highest magnification possible, because you will be able to see more detail, and you should record your observations at this magnification. Always begin with the lower magnifications, once you have found your specimen center it and focus it before increasing the magnification. We will not be using the 100X objective because it requires special handling and immersion oil, so you should use the 40X objective for most observations. What is the total magnification of a sample view with the 40X objective? Remember there is a lens in the ocular that magnifies the image 10x, so the total magnification will be 40 x 10 = 400X. Exercise 1: Examining Prokaryotic cells 1. What types of organisms are prokaryotic? 2. What structures are found in a Eukaryotic cell that will not be visible in a Prokayotic cell? 3. Examine the specimens available at the back of the classroom. Locate a prokaryotic cell and bring it back to your microscope. Name of specimen: ____________________ 4. Examine your specimen under the microscope. Sketch your specimen under 40x magnification below. Be sure to label any internal or external structures visible. Exercise 2: Examining Eukaryotic cells The cells on which we focus most of our attention are relatively large and complex, although many details of their structure are only evident with electron microscopy. The word eukaryote derives from the visible nucleus found in most of these cells. No one cell will show all of the features that a cell might have, so you will be observing a variety of eukaryotic cells. Protist cells: The protist kingdom includes eukaryotic unicellular forms (e.g., protozoans, algae, slime molds) as well as some simple multicellular forms. Many are mobile; some are photosynthetic; others are animal-like, ingesting food particles, or fungi-like, secreting digestive chemicals into the environment. Protists are diverse, often having complex cells. 1. What structures do you expect to see in these cells that was not evident in the prokaryotes you saw? Cultures of mixed protists and/or pond water are available in lab. Many different forms may be found in these cultures, including amoebas, flagellates and ciliates. (Many classifications of protists are based on how they move – with flagella, or cilia, or neither of these.) 2. Examine the specimens available at the back of the classroom. Locate the cultures of protist cells. You will examine two separate cultures. One of the cultures you’ll examine is the â€Å"Mixed Pond Protists,† you can pick which specimen you’ll examine for your second sample from the other cultures present in class. Make a wet mount of each culture, drawing material from the debris at the bottom of the culture jar. Name of specimen #1: Mixed Pond Protists Name of specimen #2: ____________________ 3. Observe first with scanning power (4X objective), looking for movement. If you don’t see anything, wash the material back into the jar and get another drop. Once you have located organisms, observe on higher power (40X objective). If the protists are moving very rapidly, they may swim out of the field of view. In this case, remove the coverslip and add a small drop of Detain. Detain is a thick solution that will help slow down the protists. REMEMBER THAT THE MICROSCOPE LIGHT IS HOT. Turn it off when you are not looking at the slide. 4. Examine your specimen under the microscope. Sketch each specimen under 40x magnification below. Be sure to label any internal or external structures visible. 5. What evidence do you see that protists are eukaryotes? 6. Attempt to identify the protists you have observed. There are several keys to protists available. Your instructor can help you use the keys if you’re not familiar with how they work. Write the name of your protists and your rationale for identification based on the characteristics in the key into your notes. When you have finished looking at your slide, place the slide in the bleach water. Plant cells: Plant cells are different from animal cells in many ways. 1. Name three ways plant cells differ from animal cells below. As in animals, cells of plants are organized into tissues, aggregations of similar cells performing a common function in a multicellular organism. In this part of the lab, you will look at several types of plant cells. Onion epidermal cells: 1. Make a wet mount of a colorless scale of an onion bulb by peeling the thin, membranous layer from the inside of one of the bulb scales and placing it on a slide in a drop of water. This usually works best if you partially slice through a ring, allowing the thin layer of tissue on the inner surface of the leaf to be pulled off. Use forceps to arrange the tissue in a drop of Iodine on a slide and cover with a cover slip. 2. Observe your preparation with the microscope, focusing first with the scanning power objective. Continue your study, switching to the low power (10 X) and finally the high power objective (40 X). 3. What shape do most of these cells have? What structure maintains this shape? 4. What structure is stained by the iodine? 5. Examine your specimen under the microscope. Sketch each specimen under 40x magnification below. Be sure to label any internal or external structures visible. 6. Which cellular components present in most leaf cells are absent from onion leaf cells? Elodea leaf cells: 1. Remove a single young leaf and prepare a wet mount. Examine the leaf first on scanning power, then on low power. 2. Examine your specimen under the microscope. Sketch each specimen under 40x magnification below. Be sure to label any internal or external structures visible. 3. After the cells have warmed a bit from the light of the microscope, you may see cytoplasmic streaming. Microfilaments in the cytoplasm are thought to be responsible for this intracellular movement. What cellular structure do you see moving around the cytoplasm? Animal cells: Human epithelial (cheek) cells: 1. Using the broad end of a clean toothpick, gently scrape the inside of your cheek. Stir the scrapings into a drop of saline and a drop of Methylene Blue on a clean microscope slide and add a cover slip. Do not reuse your toothpick. DO NOT PUT IT BACK WITH THE CLEAN TOOTHPICKS AND DON’T LAY IT ON THE TABLE. Discard it in the BIOHAZARD bag. 2. Because the cells are almost transparent, decrease the amount of light entering the objective lens to increase the contrast. Find the cells using the low power objective of your microscope, then switch to the high power objective for detailed study. Find the nucleus of a cell. Many of the cells will be folded or wrinkled due to their thin, flexible nature. 3. Examine your specimen under the microscope. Sketch each specimen under 40x magnification below. Be sure to label any internal or external structures visible. 4. Dark specs on the cells are bacteria, similar to the E. coli you looked at earlier. How big are these bacterial cells relative to your cheek cells? Discard this slide into the bowl containing the bleach water. Sheep’s blood (wet mount): Blood consists of two parts – a liquid portion (water + proteins) called plasma, and a cellular portion, containing many different cell types, including erythrocytes or red blood cells (RBCs), and leukocytes or white blood cells (WBCs). Red blood cells are red because they contain a lot of the oxygen-carrying molecule hemoglobin. The blood sample we have has been treated chemically to prevent clotting. 1. Prepare a wet mount by placing a drop of saline and a small drop of blood on the slide. Add a cover slip. Examine a portion of the slide in which the blood is diluted. 2. Examine your specimen under the microscope. Sketch each specimen under 40x magnification below. Be sure to label any internal or external structures visible. 3. What is missing from these cells that is found in eukaryotic cells? 4. How do the red blood cells compare to your cheek cells in size? What shapes are the cells? Discard this slide into the bowl containing the bleach water. Cells Review Questions Your answers should be specific and to the level of the lecture, book and lab manual. You may need to use outside sources. 1. What characteristics do all cells have in common? 2. What characteristics are common to all eukaryotic cells but not found in prokaryotic cells? 3. Would you expect that all the living cells you saw in lab contain mitochondria? Explain. 4. Did all living plant cells you observed contain chloroplasts? Explain. 5. Describe as many differences as you can between plant and animal cells at a cellular level. Do any of these differences relate to the organismic differences we see between plants and animals? Explain. 6. How do prokaryotic and eukaryotic cells compare in size? 7. How would you compare a protist cell to a cell from your body, a cheek cell for example, in terms of complexity at the cellular level?

Advertising & Consumers Essay Example | Topics and Well Written Essays - 2000 words

Advertising & Consumers - Essay Example and Trehan, R. (2011) as advertisement. According to Bishnoi and Sharma (2009), advertisement refers to a persuasive, normally paid for, impersonal communication on services, products or ideas through various media by a specific sponsor. The intention of the advertiser would be to spread ideas on the product and offerings, recognised by Belch, George Edward, Belch, George Eugene & Belch, M. (2006) as a marketers’ tool of communication to customers. Wang, Zhang and Ouyang (2009) categorise the effects of advertising as either intermediate, which influences the beliefs and attitudes of consumers or behavioural, which relates to the purchase and brand choice of consumers. Advertisement banks on psychological factors that influence consumer behaviour to reach out to the customer. These psychological factors include learning, personality and lifestyle, attitude, image and individual needs. Studying these factors would guide the advertiser on the message, ad-appeal, colour, illustra tions, media, media scheduling and the layout of the ad-copy among other critical advertisement factors. If these consumer behaviour factors would be related to the target audience, advertisers would come up with effective advertisements. Impact of advertisements on consumer behaviour ... Sutherland (2009) appreciates the importance of repetition in advertisements with consistency in messages so as to increase familiarity especially with the use of a catch line, an observation opposed by Chung and Zhao who noted that â€Å"ad repetition has no influence on perceived humour and overall effectiveness of advertising† (2003, p.121). Percy and Elliott (2012) add to this debate arguing that in direct response advertising, there would be no need for repeated advertising as action terminates with a single purchase. Consumer choices would be influenced by the brand and product image and their perception towards them (Percy & Elliot, 2012). Through advertisement, advertisers could change consumers’ perception through association of the product with attributes appealing to the needs of the consumer. Consequently, in their minds, consumers would associate that attribute to that particular brand more than they would for competitor’s brand, thus influencing pur chasing behaviour (Sutherland, 2009). Different organisations would have differences in their dependencies on advertisement to sell their products. For example, a cereal company would have to advertise more due to the existence of varied competing products as opposed to a power company which has minimal or no competition (Yeshin 2006). A strategy that advertisers employ to influence consumers to purchase products has been noted to be the creation of a feeling of lack or scarcity (Wells & Foxall, 2012). But noting the role that brands play in influencing consumption of products, Dahlen, Granlund and Grenros (2009) introduce an important role of advertising in brand

The Sentencing Phase in the United States Research Paper

The Sentencing Phase in the United States - Research Paper Example The research paper "The Sentencing Phase in the United States" deals on the sentencing phase of the criminal justice system and tries to discuss some of the more urgent issues regarding this crucial phase. The justice system forms the third leg of the triad in the handling of criminal cases, the other two being law enforcement system and the penal or corrections system. All three must work together for penal sanctions to be effective as a deterrent to the commission of crimes. The wheels of justice can indeed grind very slowly and sometimes it takes years for the victims to get vindication and justice for the harm done to them. In a sense, the concern to be so careful to avoid penalizing the innocent is the main cause of this slowness in the justice system. No criminal justice system is perfect. Be that as it may, it should not prevent society nor preclude the government from imposing justice for deviant behaviors. The idea is that the system must be geared towards fairness and equality; flaws in the system can be corrected over time and must not adversely affect the sense of trust by the people that the system is indeed fair. Lapses in the procedures, processes, and practices must not undermine the whole legal system. There are a lot of variables that can confound and confuse the litigants in many instances. A person can opt out of the criminal justice system through a plea bargain agreement that will lessen the sentence to be imposed and may subject the defendant to probation.

Money and life, Rhetorical Analysis Essay Example | Topics and Well Written Essays - 1000 words

Money and life, Rhetorical Analysis - Essay Example Logos on the other hand is the use of reason, logic or sound argument in justifying an author’s claims. With logos, the author instead convinces the audience by the soundness of his or her argument rather than appealing to the audience’s emotion. Ethos on the other hand uses ethical appeal to win over the audience to the author’s point of view. In the film, the argument is grounded mainly on the moral, ethical ascendancy of a resource person or the expertise of the person making an argument to convince the audience to believe the argument of an author. Katie Teague used a combination of these rhetorical tools in presenting her case and argument in her film Money and Life. The author used the rhetorical tool of logos heavily or the use of reason and logic in presenting her case in the film. This was evident when she argued the financial and monetary system that made â€Å"money as a mean of exchange† and theories of scarcity in the movie. In a way, she also discussed government’s monetary and fiscal policy that made money so important. The approach of the paper is also methodical and logical just like a university paper where there is a framework in laying out the arguments beginning with a statement of a problem, analysis and then a proposal of a solution. The statement of the problem was our entrapment with money and the background is Teague discussion of the monetary system that made money so important to us. First, Katie Teague laid down the general description of the â€Å"curses and evils† caused by money in the film as her statement of the problem and the byproduct of the monetary system of the country which we are all slav es. After stating the problem, the obvious comes next with the analysis of the problem of which the solution can be derived. Here, the approach of the analysis is not much dependent on logos or logic as it should be but rather on pathos or emotional appeal and ethos or credibility of the source. This

Madonna of the Meadows and Madonna of the Long Neck Essay

Madonna of the Meadows and Madonna of the Long Neck - Essay Example Raphael’s painting shows the Madonna as a softly rounded woman with soft blonde hair gathered into a soft bun out of the viewer’s angle of vision. She is delicately bent over her son who is depicted as a plump and somewhat strangely proportioned infant just on the verge of walking. He is reaching out for a wooden pole suggestive of a cross held by a slightly older St. John. This entire scene is seen over the backdrop of a very green landscape that stretches far back into the distance with a large lake and mountains visible far into the horizon. Parmigianino’s painting also depicts the Madonna tenderly holding the infant baby Jesus. She is tall and columnar as she looks down on an elongated infant who seems on the verge of falling out of her lap. She perches on a pedestal backed on one side by a rich red velvet curtain while a large grouping of angels gathers around her on this side to look adoringly at the baby. One of these angels is carrying a vase or urn that has a mirrorlike finish to it. It is positioned in such a way that it would throw a reflection back at the baby Jesus and may be the reason the baby was startled as the vase also has an etched cross on its surface. On the other side of the Madonna is a kind of landscape scene that is mostly a cloudscape. The visible sky is full of storm clouds with just a hint of blue on the horizon. There is also a tall Roman style column and the figure of St. Jerome. There are a number of ways that the two paintings are similar. They are both concerned with the relationship between Jesus’ birth and his eventual death as well as the presence of his mother in both of these events.

Critique Essay Example | Topics and Well Written Essays - 1000 words - 11

Critique - Essay Example The experimentation imitated the procedure used in outdated experiments. An outdated experiment is structured with both the dependent and independent variables, post testing and pretesting. Finally, control groups and experimental groups. The prison replication, as seen in the documentary, was a good experimentation for observational reasons and tested the theory that imprisonments can expose the most horrible characteristics in those who work in them and those imprisoned (Zimbardo). Zimbardo and his partners defined the procedures they used to measure the forces of stress, evaluated the data gathered from questionnaires, and documented the communications of the securities and convicts (Zimbardo). The two components of analysis were imitations of a prescribed organization of the securities and the inmates. The respondents were asked questions concerning the experimentation and the process of conducting the research experiment. The experiment entailed the dependent and independent variables, working explanations for simulation purposes, and testing of hypothesis (Zimbardo). This experimentation obeyed the measures used by the old-fashioned experiment. Some experiments do not have an investigational group and a control group. In the old research, research had no identifiable control group, but for the research purposes, it was assumed that the control group consisted of people who were not in jail and not exposed to such cruel environment (Zimbardo).  The experimental hypothesis was meant to measure the emotional effects of imprisonments on the entities based on the physical background or the prisoners themselves. The reason for the research was to pick healthy male individuals and discover the consequences of physical environment in an isolated environment (Zimbardo). The police arrested and took the prisoners to prison as presented in the

Her Lifestory Essay Example for Free

Her Lifestory Essay Now you can see how successful Concepcion Baylock is. At the age of 12 the young Concepcion was already away from her family and lived with her relatives in order to have a good education. Living with her relatives is not that easy, she had to do the household chores for them to let her stay, and she goes to school at the same time. At a very young age Concepcion showed her diligence in everything she does. When the time her grandparents took her, Concepcion’s life changed because they treat her nicely and they were good to her. However she still tried to help doing the chores because she wanted to thank them, in that simple way she showed her grandparents how thankful she is. As a teenager Concepcion supposed to be hanging out with her friends going to movies, parties, and outings. However she missed this, she would rather save her money that was given by her grandparents. She doesn’t even buy new things. When she was still in her senior years in high school she already worked by teaching grade school student. Later on she worked as a telephone operator in the United States Naval force in Subic. While she was working she married an irresponsible man, he just let Concepcion doing all the work. Good thing Concepcion successfully gets out of this marriage. She had a child with this unsuccessful marriage. Years later, Concepcion married again with an American who she had a four children. He is the one who helped her to put up her first business, they had a jeepney and taxi concession. When her business succeeded she put up her second business a four-storey hotel, which later on turned out to be a combined hotel, night club, and restaurant. Her ventures succeeded, but she remembers that before she married her second husband she faced many disapproval regarding in her plan f having a business. She never let this bring her down, despite she used this as challenge to pursue more. Concepcion was not still contented; she pursue her education in Manila. She managed to raise her children and run a business at the same time while she was studying. She had a very hectic schedule; she only had around four hours of sleep every day. After she finished her degree in commerce, she proceed to law, and then to a master’s in public administration, and a doctorate in commerce. Concepcion Blaylock is now a president and chairman of Diamond Motors Corporation. As an entrepreneur and a manager Conception knows how to deal with different type of people. She knew how to treat her people properly. She never hesitates to help them, especially those who showed loyalty to her and to the business. She never forgets to credit the companies who trust her. She always makes sure to maintain their trust to her. She never abused her creditor, who trusts her. Today, when Concepcion Blaylock looks back at her past she was very thankful for such an experience that she had. She was very grateful with the things that challenged her more to do more and thought her to be strong. A. Cultural Values| Manifestation of the Value| 1. Frugality (katipiran)| As a child, she saved money rather than buy new things; as an adult, did not immediately buy a car even though she can afford it. | 2. Risk taking (lakas ng loob)| She pursue her plan of having a business even though she knew that she don’t have any formal orientation in running a business. | 3. Amor Propio (utang na loob)| She never hesitates helping her staff especially those who have proven loyalty and commitment. 4. Harmonious labor management relations| She makes it to a point to compensate her people generously with profit sharing schemes and other benefits. | 5. Diligence (sipag)| At a young age, she already attributed this trait, she do the housemaid works and study at the same time. | 6. Endurance (pagkamtiisin)| She was already away with her family at an early age, for her to have a good educ ation. She never let this be a reason for not to achieve her goals, instead she makes this as her inspiration. | B. As a Filipino we were brought up by our parents to have a sense of adventure (pakikipagsapalaran) that is big help in venturing a business. Filipinos were not afraid of trying new things , we intend to be a risk taker. We usually put in our mind that God will help us in everything we do, that is a â€Å"bahala na† attitude. â€Å"Bahala na† attitude is not that bad because we do our part, but we just hope for the guidance and help of God. We Filipinos are well known of having a good relationship to people such as being family oriented and â€Å"pakikipagkapwa†. Filipinos are naturally have a close family ties. This trait is a good thing when you are in a business. Our family serves as our strength because we knew that they will be always be there to support us. They are the one who inspires us to do our best. Filipinos also exhibits the trait of being good to the people around us. This trait really plays a big role in how Filipino entrepreneur manages their people very well. Yes, the Filipino culture and values that were instill in us really help in building our entrepreneurial spirit.